Absorbance spectrum and standard curve

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As the concentration of a substance increases, its absorbance will also increase. The exact relationship between absorbance and concentration for a particular substance is determined by creating a standard curve. To create a standard curve, first measure the absorbances of a series of samples with known concentrations using a spectrophotometer (this data is provided for you below). Then, graph each data point with concentration on the x-axis and absorbance on the y-axis. Create a line of best-fit. Now you can use interpolation to estimate the concentration of an unknown sample by measuring its absorbance (absorbance values also provided below).

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